Anti-PD-1 [RMP1-30], Mouse IgG2b, kappa
AB04634-3.0
ApplicationsFlow Cytometry, Neutralisation/Blocking, Other Application
Product group Antibodies
ReactivityMouse
TargetPdcd1
Overview
- SupplierAbsolute Antibody
- Product NameAnti-PD-1 [RMP1-30], Mouse IgG2b, kappa
- Delivery Days Customer9
- Antibody SpecificityThis antibody is specific for mouse PD-1.
- Application Supplier NoteThe original version of this antibody (rat IgG2b) was used to investigate PD-1 signaling pathways in the context of allergic sensitization and challenge in mice. Specifically, it was used to determine how PD-1 blockade affects airway reactivity and immune responses (Matsumoto et al., 2004; PMID: 14764726). The original antibody was used successfully for blocking the PD-1 pathway in combination with ex vivo TKD/IL-2-stimulated NK cells in preclinical models of glioblastoma and lung cancer. It was found that this combination therapy significantly enhanced the cytotoxic activity of NK cells compared to monotherapy. The treatment resulted in improved overall survival, massive infiltration of tumors by CD8+ cytotoxic lymphocytes and NK1.1 cells, and a reduction of PD-1+ immune cells in the tumor microenvironment. Additionally, the combined therapy maintained its anti-tumor effects even after discontinuation, suggesting long-term benefits and highlighting the potential of this combined immunotherapeutic approach (Shevtsov et al., 2019; PMID: 30967859). The original version of this antibody was used successfully in an in vitro blocking assay on EL4 cells. It was also used for FC analysis and was found to stain the cells with medium intensity compared to the other tested antibodies (Polesso et al., 2021; PMID: 33684223). This antibody was also used for the FC and FACS analyses on tumor-infiltrating immune cells (TIIs) (Yokota et al., 2021; PMID: 34355173) and murine B16-F10 and YUMM melanoma cells and T-cells subsets (Martins et al., 2022; PMID: 35864188). This antibody was tested for its binding affinity to Jurkat cells expressing mouse PD-1. It exhibited intermediate avidity for PD-1 and showed limited capability to reverse PD-1-mediated T-cell inhibition compared to other tested antibodies. It demonstrated partial epitope blocking against itself and another PD-1 monoclonal antibody, indicating its suitability for specific experimental conditions requiring PD-1 staining alongside functional assessments (Bu et al., 2022; PMID: 35925787).
- ApplicationsFlow Cytometry, Neutralisation/Blocking, Other Application
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDRMP1-30
- Gene ID18566
- Target namePdcd1
- Target descriptionprogrammed cell death 1
- Target synonymsLy101; mPD-1; PD-; PD-1; Pdc; Pdc1; programmed cell death 1 protein; programmed cell death protein 1; protein PD-1
- HostMouse
- IsotypeIgG2b
- Protein IDQ02242
- Protein NameProgrammed cell death protein 1
- ReactivityMouse
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203