Anti-PD-1H [MH5A]

Absolute Antibody

Anti-PD-1H [MH5A]

9

This antibody specifically binds PD-1H.

The antibody has been labelled with biotin and used to stain formalin-fixed tissues from wild-type C57BL/6 mice at 6 weeks of age. This showed specific staining for PD-1H in the T cell zone and marginal zone. The antibody stains P815 cells transfected with full-length PD-1H plasmid, but not control plasmid. Antibody specificity has been confirmed by ELISA. A single 200-microg dose of MH5A prevented aGVHD in mouse models, caused a reduction in accumulation and expansion of CD8+ and CD4+ T cells in spleens and livers and greatly reduced numbers of inflitrating T cells in all aGVHD target tissues examined. Few mAbs targeting a single cosignalling molecule have such a potent preventative effect (Flies D et al, 2011).

ELISA, ImmunoHistoChemistry, Neutralisation/Blocking

Research Use Only

Monoclonal

MH5A

Vsir

V-set immunoregulatory receptor

4632428N05Rik; Die; Dies1; differentiation of ESCs 1; PD-1; PD-1H; platelet receptor Gi24; V; VISTA; V-set domain-containing immunoregulatory receptor; V-type immunoglobulin domain-containing suppressor of T-cell activation

Mouse

IgG2a

V-type immunoglobulin domain-containing suppressor of T-cell activation

This chimeric mouse antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.

Mouse

-20°C,2°C to 8°C

12352203