Anti-PD-1H [MH5A]

Absolute Antibody

Anti-PD-1H [MH5A]

7

The antibody has been labelled with biotin and used to stain formalin-fixed tissues from wild-type C57BL/6 mice at 6 weeks of age. This showed specific staining for PD-1H in the T cell zone and marginal zone. The antibody stains P815 cells transfected with full-length PD-1H plasmid, but not control plasmid. Antibody specificity has been confirmed by ELISA. A single 200-microg dose of MH5A prevented aGVHD in mouse models, caused a reduction in accumulation and expansion of CD8+ and CD4+ T cells in spleens and livers and greatly reduced numbers of inflitrating T cells in all aGVHD target tissues examined. Few mAbs targeting a single cosignalling molecule have such a potent preventative effect (Flies D et al, 2011).

ELISA, ImmunoHistoChemistry, Neutralisation/Blocking

ELISA; IHC; block; therapeutic

Research Use Only

V-type immunoglobulin domain-containing suppressor of T-cell activation

This chimeric mouse antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.

-20°C,2°C to 8°C

12352203