Anti-PD-L1 [JC071]

Absolute Antibody

Anti-PD-L1 [JC071]

9

This antibody binds canine PD-L1.

This antibody is recommended for detection of canine PD-L1. This antibody successfully detected canine PD-L1 in a western blot assay. JC071 was used to determine the increased PD-1 expression on canine PBMCs stimulated with peptidoglycan or lipopolysaccharide using flow cytometry. JC071 was also able to detect recombinant PD-L1 expressed on DH82 cell line both with and without in vitro activation using IFN-gamma in flow cytometry. It also recognized recombinant canine PD-L1 expressed on CHO cells in flow cytometry. The ability of the antibody to block interactions between PD-1 and PD-L1 was checked using ELISA. It is known that blockade of PD-L1 and PD-1 interactions causes increase in Ifn-gamma level. JC071 successfully blocked PD-L1 and amplified IFN-gamma production in culture by approximately 6-fold. Thus, JC071 alleviated T-cell suppression and led to enhanced functional activity of T cells in culture and have potential application as an immunotherapy reagent for cancer treatment (PMID: 32614928).

Flow Cytometry, Western Blot, ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

JC071

CD274

CD274 molecule

PDL1; PD-L1; programmed cell death 1 ligand 1; programmed death ligand 1

Rabbit

IgG

CD274 molecule

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Canine

-20°C,2°C to 8°C

12352203