Figure 1. Western blot analysis of PD1/PDCD1 using anti-PD1/PDCD1 antibody (RP1039). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human COLO-320 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse RAW246.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PD1/PDCD1 antigen affinity purified polyclonal antibody (Catalog # RP1039) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PD1/PDCD1 at approximately 65KD. The expected band size for PD1/PDCD1 is at 32KD.
Figure 1. Western blot analysis of PD1/PDCD1 using anti-PD1/PDCD1 antibody (RP1039). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human COLO-320 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse RAW246.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PD1/PDCD1 antigen affinity purified polyclonal antibody (Catalog # RP1039) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PD1/PDCD1 at approximately 65KD. The expected band size for PD1/PDCD1 is at 32KD.
Anti-PD1/PDCD1 Antibody Picoband(r)
RP1039-CY3
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetPDCD1
Overview
- SupplierBoster Bio
- Product NameAnti-PD1/PDCD1 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteWB: The detection limit for PD1 is approximately 0.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateCy3
- Gene ID5133
- Target namePDCD1
- Target descriptionprogrammed cell death 1
- Target synonymsCD279; hPD-1; hPD-l; hSLE1; PD1; PD-1; programmed cell death 1 protein; programmed cell death protein 1; protein PD-1; SLEB2; systemic lupus erythematosus susceptibility 2
- HostRabbit
- IsotypeIgG
- Protein IDQ15116
- Protein NameProgrammed cell death protein 1
- Scientific DescriptionBoster Bio Anti-PD1/PDCD1 Antibody catalog # RP1039. Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203