Figure 1. Western blot analysis of Pea3 using anti-Pea3 antibody (PB9699). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Lung Tissue Lysate at 50ug, Lane 2: HELA Whole Cell Lysate at 40ug, Lane 3: A549 Whole Cell Lysate at 40ug, Lane 4: SW620 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Pea3 antigen affinity purified polyclonal antibody (Catalog # PB9699) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Pea3 at approximately 68 kDa. The expected band size for Pea3 is at 54 kDa.
Figure 1. Western blot analysis of Pea3 using anti-Pea3 antibody (PB9699). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Lung Tissue Lysate at 50ug, Lane 2: HELA Whole Cell Lysate at 40ug, Lane 3: A549 Whole Cell Lysate at 40ug, Lane 4: SW620 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Pea3 antigen affinity purified polyclonal antibody (Catalog # PB9699) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Pea3 at approximately 68 kDa. The expected band size for Pea3 is at 54 kDa.
Anti-Pea3/ETV4 Antibody Picoband(r)
PB9699
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Rat
TargetETV4
Overview
- SupplierBoster Bio
- Product NameAnti-Pea3 Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID2118
- Target nameETV4
- Target descriptionETS variant transcription factor 4
- Target synonymsadenovirus E1A enhancer-binding protein; E1AF; E1A-F; ETS translocation variant 4; ETS variant 4; ets variant gene 4 (E1A enhancer-binding protein, E1AF); EWS protein/E1A enhancer binding protein chimera; PEA3; PEAS3; polyomavirus enhancer activator 3 homolog
- HostRabbit
- IsotypeIgG
- Protein IDP43268
- Protein NameETS translocation variant 4
- Scientific DescriptionBoster Bio Anti-Pea3/ETV4 Antibody Picoband® catalog # PB9699. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203