Figure 4. IHC analysis of Peptide YY using anti-Peptide YY antibody (PB10014). Peptide YY was detected in paraffin-embedded section of mouse pancreas tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-Peptide YY Antibody (PB10014) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 4. IHC analysis of Peptide YY using anti-Peptide YY antibody (PB10014). Peptide YY was detected in paraffin-embedded section of mouse pancreas tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-Peptide YY Antibody (PB10014) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-Peptide YY/PYY Antibody
PB10014-DYLIGHT488
ApplicationsImmunoFluorescence, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse, Rat
TargetPyy
Overview
- SupplierBoster Bio
- Product NameAnti-Peptide YY/PYY Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsImmunoFluorescence, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateDyLight 488
- Gene ID217212
- Target namePyy
- Target descriptionpeptide YY
- Target synonymsPeptide tyrosine tyrosine; peptide YY
- HostRabbit
- IsotypeIgG
- Protein IDQ9EPS2
- Protein NamePeptide YY
- Scientific DescriptionBoster Bio Anti-Peptide YY/PYY Antibody Picoband® catalog # PB10014. Tested in IF, IHC applications. This antibody reacts with Mouse, Rat.
- ReactivityMouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203