Figure 1. Western blot analysis of Periostin using anti-Periostin antibody (A01378). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat gaster tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: rat NRK whole cell lysates, Lane 4: mouse gaster tissue lysates, Lane 5: mouse intestine tissue lysates, Lane 6: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Periostin antigen affinity purified polyclonal antibody (Catalog # A01378) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Periostin at approximately 93KD. The expected band size for Periostin is at 93KD.
Figure 1. Western blot analysis of Periostin using anti-Periostin antibody (A01378). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat gaster tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: rat NRK whole cell lysates, Lane 4: mouse gaster tissue lysates, Lane 5: mouse intestine tissue lysates, Lane 6: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Periostin antigen affinity purified polyclonal antibody (Catalog # A01378) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Periostin at approximately 93KD. The expected band size for Periostin is at 93KD.
Anti-Periostin/Postn Antibody Picoband(r)
A01378
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse, Rat
TargetPostn
Overview
- SupplierBoster Bio
- Product NameAnti-Periostin Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID361945
- Target namePostn
- Target descriptionperiostin
- Target synonymsperiostin; periostin, osteoblast specific factor; periostin-like factor; Plf
- HostRabbit
- IsotypeIgG
- Protein IDD3ZAF5
- Protein NamePeriostin, osteoblast specific factor (Predicted), isoform CRA_a
- Scientific DescriptionBoster Bio Anti-Periostin/Postn Antibody Picoband® catalog # A01378. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityMouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203