Figure 4. IHC analysis of Peroxiredoxin 4 using anti-Peroxiredoxin 4 antibody (PB9383). Peroxiredoxin 4 was detected in paraffin-embedded section of mouse Brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-Peroxiredoxin 4 Antibody (PB9383) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 4. IHC analysis of Peroxiredoxin 4 using anti-Peroxiredoxin 4 antibody (PB9383). Peroxiredoxin 4 was detected in paraffin-embedded section of mouse Brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-Peroxiredoxin 4 Antibody (PB9383) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-Peroxiredoxin 4/PRDX4 Antibody Picoband(r)
PB9383-BIOTIN
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPRDX4
Overview
- SupplierBoster Bio
- Product NameAnti-Peroxiredoxin 4/PRDX4 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteWB: The detection limit for Peroxiredoxin 4 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateBiotin
- Gene ID10549
- Target namePRDX4
- Target descriptionperoxiredoxin 4
- Target synonymsantioxidant enzyme AOE372; AOE372; AOE37-2; epididymis secretory sperm binding protein Li 97n; HEL-S-97n; peroxiredoxin IV; peroxiredoxin-4; PRX-4; prx-IV; thioredoxin peroxidase (antioxidant enzyme); thioredoxin peroxidase AO372; thioredoxin-dependent peroxide reductase A0372; thioredoxin-dependent peroxiredoxin 4
- HostRabbit
- IsotypeIgG
- Protein IDQ13162
- Protein NamePeroxiredoxin-4
- Scientific DescriptionBoster Bio Anti-Peroxiredoxin 4/PRDX4 Antibody Picoband® catalog # PB9383. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203