Anti-PERV [A5]

Absolute Antibody

Anti-PERV [A5]

7

This antibody recognizes and binds the gag polyprotein and specifically recognizes the amino acids between 47 and 113 of p15 matrix protein of the porcine endogenous retrovirus.

This variable single heavy chain antibody to 60kDa gag polyprotein and p15 matrix protein of the PERV and its binding was confirmed by Western blotting. The antibody has shown to reduce the (PERV-A/B) virus production when it is intracellularly expressed in PK cells. This antibody with its high affinity for p15 matrix protein of the PERV gag polyprotein, solubility in aqueous environments and intracellular expression shows potential to be used as an intrabody to block the production of PERV and prevent risk of infections or transmission of infectious diseases (xenozoonosis) when tissues and organs from genetically modified pigs are to be used in xenotransplantation (Dekker et al., 2003).

Western Blot

WB

Research Use Only

Monoclonal

A5

Mouse

IgG1

This chimeric mouse antibody was made using the variable domain sequences of the original VHH format, for improved compatibility with existing reagents, assays and techniques.

Virus

Porcine Endogenous Retrovirus

This variable heavy chain fragment was prepared by immunization of a young adult male Llama glama with a purified 60-kDa PERV-B gag protein. The gag protein was prepared by amplifying PERV-B gag cDNA from PK15 cell RNA using a forward and reverse primer and later on cloning it into the pET-30a expression vector.

-20°C,2°C to 8°C

12352203