Figure 1. Western blot analysis of PGBD4 using anti-PGBD4 antibody (A19178-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGBD4 antigen affinity purified polyclonal antibody (Catalog # A19178-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PGBD4 at approximately 70 kDa. The expected band size for PGBD4 is at 67 kDa.
Figure 1. Western blot analysis of PGBD4 using anti-PGBD4 antibody (A19178-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGBD4 antigen affinity purified polyclonal antibody (Catalog # A19178-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PGBD4 at approximately 70 kDa. The expected band size for PGBD4 is at 67 kDa.
Anti-PGBD4 Antibody Picoband(r)
A19178-1-PE
TargetPGBD4
Product group Antibodies
Overview
- SupplierBoster Bio
- Product NameAnti-PGBD4 Antibody Picoband(r)
- Delivery Days Customer9
- Concentration500 ug/ml
- Gene ID161779
- Target namePGBD4
- Target descriptionpiggyBac transposable element derived 4
- Target synonymspiggyBac transposable element-derived protein 4
- Protein IDQ96DM1
- Protein NamePiggyBac transposable element-derived protein 4
- Scientific DescriptionBoster Bio Anti-PGBD4 Antibody Picoband® catalog # A19178-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203