Anti-Pharynx [KT22]

Absolute Antibody

Anti-Pharynx [KT22]

7

This antibody recognized the apical side of the pharynx of Caenorhabditis elegans. Caenorhabditis elegans is a transparent nematode that lives in temperate soil environments.

This antibody was used for the staining of the apical region of the pharynx of Caenorhabditis elegans. The KT22 signal was detectable in the twofold embryo as a single line from the buccal cavity to the end of isthmus; at the pretzel stage, the KT22 stained three lines from the buccal cavity to the anterior isthmus, which appeared to merge into a single line in the posterior isthmus region. No KT22 signal was detected in the terminal bulb and pharyngeal-intestinal valve (PMID: 18498356).

ImmunoFluorescence

IF;

Research Use Only

Monoclonal

KT22

Rabbit

IgG

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgM format for improved compatibility with existing reagents assays and techniques.

C. Elegans

Caenorhabditis elegans

The original antibody was generated by immunizing BALB/c mice with subtracted embryonic extract. Subtracted extracts were prepared by using a method called “antigen subtraction” which enriched antigens of low abundance in heterogeneous molecule mixtures by subtracting abundant antigens.

-20°C,2°C to 8°C

12352203