Immunohistochemical analysis of paraffin-embedded human pancreas, using Phospho-Smad5 (S463/465) Antibody(P01423-1) SMAD5 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SMAD5 Antibody (P01423-1)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Immunohistochemical analysis of paraffin-embedded human pancreas, using Phospho-Smad5 (S463/465) Antibody(P01423-1) SMAD5 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SMAD5 Antibody (P01423-1)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-Phospho-Smad5 (S463/465) Rabbit Monoclonal Antibody
P01423-1
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSMAD5
Overview
- SupplierBoster Bio
- Product NameAnti-Phospho-Smad5 (S463/465) Rabbit Monoclonal Antibody
- Delivery Days Customer9
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDAFB-19
- FormulationLiquid
- Gene ID4090
- Target nameSMAD5
- Target descriptionSMAD family member 5
- Target synonymsDWFC; JV5-1; MAD, mothers against decapentaplegic homolog 5; MADH5; mothers against decapentaplegic homolog 5; mothers against decapentaplegic, drosophila, homolog of, 5; SMA- and MAD-related protein 5; SMAD, mothers against DPP homolog 5
- HostRabbit
- IsotypeIgG
- Modification TypePhosphorylated
- Protein IDQ99717
- Protein NameMothers against decapentaplegic homolog 5
- Scientific DescriptionBoster Bio Anti-Phospho-Smad5 (S463/465) Rabbit Monoclonal Antibody catalog # P01423-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203