Anti-Phosphovimentin [4A4]

Absolute Antibody

Anti-Phosphovimentin [4A4]

9

This antibody specifically binds mouse and human vimentin phosphorylated at serine 55 by cdc2 kinase . This antibody is incapable of binding non-phosphorylated vimentin or with vimentin phosphorylated by other kinases.

This antibody was developed to investigate the role of intermediate filament (IF) protein phosphorylation by cdc2 kinase during mitosis. The binding specificity of 4A4 towards serine 55 phosphorylated vimentin was determined by ELISA. Western blotting indicated that this antibody reacted with vimentin phosphorylated by cdc2 kinase but not with non-phosphorylated vimentin or with vimentin phosphorylated by other kinases such as CAMP-dependent protein kinase, protein kinase C, or Ca2+-calmodulin-dependent protein kinase 11. Immunofluorescence and immunoelectron microscopy showed that vimentin Ser55 residues distributed in the entire cytoplasmic vimentin filament system are phosphorylated when the cells enter mitosis and dephosphorylated in cytokinesis (PMID: 7983050). This antibody was also used in the visualization of mitotic radial glial lineage cells in the developing rat brain which revealed different patterns of gliogenesis in the neocortex and cerebellum (PMID: 9633804). This antibody has been commonly used for the visualization and study of phosphorylation of vimentin during mitosis (PMID: 8601602, 8992382, 9565595).

ImmunoFluorescence, Western Blot, ELISA

Research Use Only

Monoclonal

4A4

Vim

vimentin

vimentin

Mouse

IgG2b

Vimentin

Human, Mouse

-20°C,2°C to 8°C

12352203