Figure 1. Western blot analysis of PIAS4 using anti-PIAS4 antibody (PB10082). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates, Lane 3: HELA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIAS4 antigen affinity purified polyclonal antibody (Catalog # PB10082) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIAS4 at approximately 57 kDa. The expected band size for PIAS4 is at 57 kDa.
Figure 1. Western blot analysis of PIAS4 using anti-PIAS4 antibody (PB10082). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates, Lane 3: HELA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIAS4 antigen affinity purified polyclonal antibody (Catalog # PB10082) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIAS4 at approximately 57 kDa. The expected band size for PIAS4 is at 57 kDa.
Anti-E3 SUMO-protein ligase PIAS4 Antibody Picoband(r)
PB10082
ApplicationsWestern Blot
Product group Antibodies
ReactivityBovine, Canine, Equine, Human, Monkey, Mouse, Rat
TargetPIAS4
Overview
- SupplierBoster Bio
- Product NameAnti-PIAS4 Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID51588
- Target namePIAS4
- Target descriptionprotein inhibitor of activated STAT 4
- Target synonymsE3 SUMO-protein ligase PIAS4; Piasg; PIAS-gamma; PIASY; protein inhibitor of activated STAT protein 4; protein inhibitor of activated STAT protein gamma; protein inhibitor of activated STAT protein PIASy; RING-type E3 ubiquitin transferase PIAS4; zinc finger, MIZ-type containing 6; ZMIZ6
- HostRabbit
- IsotypeIgG
- Protein IDQ8N2W9
- Protein NameE3 SUMO-protein ligase PIAS4
- Scientific DescriptionBoster Bio Anti-E3 SUMO-protein ligase PIAS4 Antibody Picoband® catalog # PB10082. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityBovine, Canine, Equine, Human, Monkey, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203