IHC analysis of PIK3R2 using anti-PIK3R2 antibody (PA1680). PIK3R2 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PIK3R2 Antibody (PA1680) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
IHC analysis of PIK3R2 using anti-PIK3R2 antibody (PA1680). PIK3R2 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PIK3R2 Antibody (PA1680) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-PI 3 Kinase p85 beta/PIK3R2 Antibody Picoband(r)
PA1680
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHamster, Human, Mouse, Rat
TargetPIK3R2
Overview
- SupplierBoster Bio
- Product NameAnti-PIK3R2 Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ImmunoHistoChemistry
- Applications SupplierIHP, WB, IHC
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID5296
- Target namePIK3R2
- Target descriptionphosphoinositide-3-kinase regulatory subunit 2
- Target synonymsMPPH; MPPH1; p85; P85B; p85-BETA; phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta; phosphatidylinositol 3-kinase regulatory subunit beta; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 2 (p85 beta); phosphoinositide-3-kinase regulatory subunit beta; phosphoinositide-3-kinase, regulatory subunit 2 (beta); PI3K regulatory subunit beta; PI3-kinase subunit p85-beta; ptdIns-3-kinase regulatory subunit p85-beta
- HostRabbit
- IsotypeIgG
- Protein IDO00459
- Protein NamePhosphatidylinositol 3-kinase regulatory subunit beta
- Scientific DescriptionBoster Bio Anti-PI 3 Kinase p85 beta/PIK3R2 Antibody catalog # PA1680. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHamster, Human, Mouse, Rat
- Reactivity SupplierHuman, Mouse, Rat, Hamster
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203