Figure 1. Western blot analysis of PINX1 using anti-PINX1 antibody (PB9498). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Human Placenta Tissue Lysate at 50ug, Lane 2: HELA Whole Cell Lysate at 40ug, Lane 3: HUT Whole Cell Lysate at 40ug, Lane 4: JURKAT Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PINX1 antigen affinity purified polyclonal antibody (Catalog # PB9498) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PINX1 at approximately 37 kDa. The expected band size for PINX1 is at 37 kDa.
Figure 1. Western blot analysis of PINX1 using anti-PINX1 antibody (PB9498). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Human Placenta Tissue Lysate at 50ug, Lane 2: HELA Whole Cell Lysate at 40ug, Lane 3: HUT Whole Cell Lysate at 40ug, Lane 4: JURKAT Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PINX1 antigen affinity purified polyclonal antibody (Catalog # PB9498) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PINX1 at approximately 37 kDa. The expected band size for PINX1 is at 37 kDa.
Anti-PINX1 Antibody Picoband(r)
PB9498
ApplicationsWestern Blot
Product group Antibodies
ReactivityBovine, Human
TargetPINX1
Overview
- SupplierBoster Bio
- Product NameAnti-PINX1 Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID54984
- Target namePINX1
- Target descriptionPIN2 (TERF1) interacting telomerase inhibitor 1
- Target synonyms67-11-3 protein; Gno1; hepatocellular carcinoma-related putative tumor suppressor; liver-related putative tumor suppressor; LPTL; LPTS; PIN2/TERF1-interacting telomerase inhibitor 1; PIN2-interacting protein 1; pin2-interacting protein X1; protein 67-11-3; Pxr1; TRF1-interacting protein 1
- HostRabbit
- IsotypeIgG
- Protein IDQ96BK5
- Protein NamePIN2/TERF1-interacting telomerase inhibitor 1
- Scientific DescriptionBoster Bio Anti-PINX1 Antibody Picoband® catalog # PB9498. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityBovine, Human
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203