Anti-PKA-RIB (Protein Kinase-A Regulatory Subunit Ib)

Applied Biological Materials

Anti-PKA-RIB (Protein Kinase-A Regulatory Subunit Ib)

9

The antibody binds to PKARIb, but does not cross react with the other PKA regulatory subunits (PKARIa, PKARIIa, PKARIIb).

Western Blot

Research Use Only

Polyclonal

1 ug/ul

Rabbit

IgG

The antibody is a rabbit antibody to the regulatory subunit Ib of protein kinase-A, made with a synthetic peptide based on the rat sequence. The antibody binds to PKARIb, but does not cross react with the other PKA regulatory subunits (PKARIa, PKARIIa, PKARIIb). The antibody has been peptide-affinity purified, concentrated to 1 mg/ml, with the addition of Sodium Azide as preservative, and 50% glycerol as as cryoprotectant. Protein KinaseA is a critical kinase involved in numerous biological functions. The regulatory subunits of PKA allow a range of modifying agents to influence PKA activity and localization, and are found in different levels in a tissue-specific manner. RP1PKARIb works well for Western Blotting applications, and when used against the reduced protein identifies bands at 55 kD and 53 kD, as well as activation/breakdown products_recover. The antibody can recognize native or reduced PKARIb, and has application for immunoprecipitation, immunohistochemistry, cell sorting and ELISA. RP1PKARIb recognized human, rat, mouse and rabbit PKARIb. A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates. Higher concentration of antibody may be needed for non-human samples. FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.

Human, Mouse, Rat, Other Species

-20°C,2°C to 8°C

12352203