Figure 1. Western blot analysis of PKR/EIF2AK2 using anti-PKR/EIF2AK2 antibody (PB9803). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human PANC-1 whole cell lysates, Lane 6: human A431 whole cell lysates, Lane 7: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKR/EIF2AK2 antigen affinity purified polyclonal antibody (Catalog # PB9803) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKR/EIF2AK2 at approximately 69 kDa. The expected band size for PKR/EIF2AK2 is at 62 kDa.
Figure 1. Western blot analysis of PKR/EIF2AK2 using anti-PKR/EIF2AK2 antibody (PB9803). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human PANC-1 whole cell lysates, Lane 6: human A431 whole cell lysates, Lane 7: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKR/EIF2AK2 antigen affinity purified polyclonal antibody (Catalog # PB9803) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKR/EIF2AK2 at approximately 69 kDa. The expected band size for PKR/EIF2AK2 is at 62 kDa.
Anti-PKR/EIF2AK2 Antibody Picoband(r)
PB9803-BIOTIN
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetEIF2AK2
Overview
- SupplierBoster Bio
- Product NameAnti-PKR/EIF2AK2 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateBiotin
- Gene ID5610
- Target nameEIF2AK2
- Target descriptioneukaryotic translation initiation factor 2 alpha kinase 2
- Target synonymsdouble stranded RNA activated protein kinase; eIF-2A protein kinase 2; EIF2AK1; interferon-induced, double-stranded RNA-activated protein kinase; interferon-inducible elF2alpha kinase; LEUDEN; P1/eIF-2A protein kinase; p68 kinase; PKR; PPP1R83; PRKR; protein kinase R; protein kinase, interferon-inducible double stranded RNA dependent; protein phosphatase 1, regulatory subunit 83; tyrosine-protein kinase EIF2AK2
- HostRabbit
- IsotypeIgG
- Protein IDP19525
- Protein NameInterferon-induced, double-stranded RNA-activated protein kinase
- Scientific DescriptionBoster Bio Anti-PKR/EIF2AK2 Antibody Picoband® catalog # PB9803. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203