Anti-PLA2, Fraction 19 [TPL004_01_A11]

Absolute Antibody

Anti-PLA2, Fraction 19 [TPL004_01_A11]

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TPL004_01_A11 antibody has a very high affinity to the PLA2. It was demonstrated by normalized ELISA, which circumvents the limitation of a simple ELISA by removing the expression variable, normalizing the amount of scFv in each well. The antibodys high affinity was confirmed by its ability to bind the target protein in a venom fraction even at 1:32,000 dilution (3.125 10^-2) microg/mL (Juergensen et al., 2019 [poster]). It is recommended for the research on the antitoxin against Naja nigricollis snakebites.

ELISA

ELISA

Research Use Only

Monoclonal

TPL004_01_A11

Mouse

IgM

This full-length, chimeric mouse antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.

Naja nigricollis

The antibody was raised using IONTAS phage display library, a naïve human scFv library consisting of 4.0 · 10^10 unique antibody fragments. Through phage display selection, scFv-presenting phages were allowed to bind the venom proteins, phages with unspecific binding were washed away, and the rest were eluted through enzymatic digestion. These were then amplified and used for the following round. In total three rounds of selections were completed leaving the best binders behind.

-20°C,2°C to 8°C

12352203