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Immunohistochemical analysis of paraffin-embedded human melanoma, using PMEL17 / GP100 Antibody(M01262) PMEL was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PMEL Antibody (M01262)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Immunohistochemical analysis of paraffin-embedded human melanoma, using PMEL17 / GP100 Antibody(M01262) PMEL was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PMEL Antibody (M01262)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Immunohistochemical analysis of paraffin-embedded human melanoma, using PMEL17 / GP100 Antibody(M01262) PMEL was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PMEL Antibody (M01262)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Anti-PMEL17 / GP100 Rabbit Monoclonal Antibody

Research Use Only
M01262
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPMEL
Price on request
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-PMEL17 / GP100 Rabbit Monoclonal Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    AHH-16
  • Formulation
    Liquid
  • Gene ID6490
  • Target name
    PMEL
  • Target description
    premelanosome protein
  • Target synonyms
    D12S53E; gp100; ME20; ME20M; ME20-M; melanocyte protein mel 17; melanocyte protein PMEL; melanocyte protein Pmel 17; melanocytes lineage-specific antigen GP100; melanoma-associated ME20 antigen; melanosomal matrix protein17; P1; P100; PMEL17; SI; SIL; SILV; silver locus protein homolog; silver, mouse, homolog of
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP40967
  • Protein Name
    Melanocyte protein PMEL
  • Scientific Description
    Boster Bio Anti-PMEL17 / GP100 Rabbit Monoclonal Antibody catalog # M01262. Tested in WB, IHC applications. This antibody reacts with Human.
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203