Immunohistochemical analysis of paraffin-embedded human melanoma, using PMEL17 / GP100 Antibody(M01262) PMEL was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PMEL Antibody (M01262)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-PMEL17 / GP100 Rabbit Monoclonal Antibody
M01262
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPMEL
Overview
- SupplierBoster Bio
- Product NameAnti-PMEL17 / GP100 Rabbit Monoclonal Antibody
- Delivery Days Customer9
- ApplicationsWestern Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDAHH-16
- FormulationLiquid
- Gene ID6490
- Target namePMEL
- Target descriptionpremelanosome protein
- Target synonymsD12S53E; gp100; ME20; ME20M; ME20-M; melanocyte protein mel 17; melanocyte protein PMEL; melanocyte protein Pmel 17; melanocytes lineage-specific antigen GP100; melanoma-associated ME20 antigen; melanosomal matrix protein17; P1; P100; PMEL17; SI; SIL; SILV; silver locus protein homolog; silver, mouse, homolog of
- HostRabbit
- IsotypeIgG
- Protein IDP40967
- Protein NameMelanocyte protein PMEL
- Scientific DescriptionBoster Bio Anti-PMEL17 / GP100 Rabbit Monoclonal Antibody catalog # M01262. Tested in WB, IHC applications. This antibody reacts with Human.
- ReactivityHuman
- Storage Instruction-20°C
- UNSPSC12352203