Flow Cytometry analysis of A431 cells using anti-PML antibody (PB10084). Overlay histogram showing A431 cells stained with PB10084 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PML Antibody (PB10084,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Flow Cytometry analysis of A431 cells using anti-PML antibody (PB10084). Overlay histogram showing A431 cells stained with PB10084 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PML Antibody (PB10084,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Anti-PML Protein Antibody Picoband(r)
PB10084
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPML
Overview
- SupplierBoster Bio
- Product NameAnti-PML Protein Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID5371
- Target namePML
- Target descriptionPML nuclear body scaffold
- Target synonymsE3 SUMO-protein ligase PML; MYL; PML/RARA fusion; PP8675; probable transcription factor PML; promyelocytic leukemia protein; promyelocytic leukemia, inducer of; protein PML; RING finger protein 71; RING-type E3 SUMO transferase PML; RNF71; TRIM19; tripartite motif protein TRIM19; tripartite motif-containing protein 19
- HostRabbit
- IsotypeIgG
- Protein IDP29590
- Protein NameProtein PML
- Scientific DescriptionBoster Bio Anti-PML Protein Antibody Picoband® catalog # PB10084. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203