Anti-Polyglutamine [1C2]

Absolute Antibody

Anti-Polyglutamine [1C2]

9

This antibody bind the extended polyglutamine repeats found in the TATA binding protein. It is reported that the affinity of this mAb is proportional to the length of the polyglutamine chain and that this antibody recognizes polyQ stretches with 11, 22 an

This antibody recognizes recombinant and endogenous HeLa cell TBP in Western blots. Epitope mapping of the antibody was done using ELISA by incubating the mAbs with synthetic overlapping peptides having 150 amino acids from the N-terminal of the HeLa cell TBP. Thia antibody immunoprecipitates natibe TFIID from phosphocellulose 1 M KCl fractions indicating that the N-terminal region of TBP is not blocked by TBP binding proteins. It is reported that mAb 1C2 selectively inhibited in vitro transcription from TATA-containing, but not from TATA-less promoters, irrespective of whether they were transcribed by Pol II or Pol III (PMID:7510635). This antibody is reported to successfully recognize the polyglutamine chains of mutated alleles of protein from several human neurodegenerative diseases caused by a CAG repeat expansion, like Huntingtons disease and spinocerebellar ataxia type 2, 3 and 7 (PMID: 7477379). It is reported that this antibody recognises the linear and extended conformations of polyglutamine (PMID: 23777629).

ImmunoPrecipitation, Western Blot, ELISA

Research Use Only

Monoclonal

1C2

TBP

TATA-box binding protein

GTF2D; GTF2D1; HDL4; SCA17; TATA sequence-binding protein; TATA-box binding protein N-terminal domain; TATA-box factor; TATA-box-binding protein; TFIID; transcription initiation factor TFIID TBP subunit

Mouse

TATA-box-binding protein

Human

-20°C,2°C to 8°C

12352203