Anti-porcine VCAM [2A2]

Absolute Antibody

Anti-porcine VCAM [2A2]

9

This antibody is specific for an epitope within domain 1 of porcine VCAM, which is expressed on activated endothelial cells. It des not cross-react with human VCAM.

The ability of this antibody to recognise porcine VCAM has been confirmed in ELISA analysis (Mueller et al, 1995). In FC analysis, this antibody reacts with porcine VCAM on TNF-a stimulated porcine aortic endothelial cells, but not to human VCAM on TNF-a stimulated human umbilical vein endothelial cells (Mueller et al, 1995). This antibody blocks VLA-4 dependent adhesion of peripheral blood human T cells to activated porcine aortic endothelial cells (Mueller et al, 1995). It also inhibits the binding of Ramos cells to a recombinant secreted form of porcine VCAM (Mueller et al, 1995).

Flow Cytometry, ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

2A2

Rabbit

IgG

Vascular cell adhesion molecule 1

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse format, for improved compatibility with existing reagents, assays and techniques.

Porcine

-20°C,2°C to 8°C

12352203