Bio-Connect

Anti-Potato cyst nematode [MGR48]

Ab00961-1.1
Absolute Antibody
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot
Product group Antibodies
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Potato cyst nematode [MGR48]
  • Delivery Days Customer
    7
  • Application Supplier Note
    MGR48 has been used in Western Blot analyses in order to confirm the presence of secretory components in collected G. rostochiesis material (Robertson 1999). Further, MGR has been employed to immunopurify the endoglucanases from G. rostochiensis and H. glycines (Smant 1998). MGR48 has also been used in immunofluorescence testing to compare DMT-treated and nontreated preparasitic PRD-hatched J2 (Smant 1997).
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot
  • Applications Supplier
    IF; WB; IP
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MGR48
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity Supplier
    Potato cyst nematode
  • Reactivity Supplier Note
    Sixteen mice were immunised intraperitoneally with isolated protein fractions from homogenised preparasitic second-stage juveniles of G. rostochiensis, which were mixed 1:1 with Freund's incomplete adjuvant. weeks later antiserum samples were collected for immunofluorescence microscopy. One mouse that was selected for monoclonal antibody production received a final booster injection 12 weeks after the first immunization. Three days later the mouse was sacrificed, and MAb-producing hybridoma cell lines were obtained by fusing spleen cells with SP 2/0 myeloma cells.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203