Figure 1. Western blot analysis of PP11/ENDOU using anti-PP11/ENDOU antibody (A07350-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PP11/ENDOU antigen affinity purified polyclonal antibody (Catalog # A07350-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PP11/ENDOU at approximately 47 kDa. The expected band size for PP11/ENDOU is at 47 kDa.
Figure 1. Western blot analysis of PP11/ENDOU using anti-PP11/ENDOU antibody (A07350-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PP11/ENDOU antigen affinity purified polyclonal antibody (Catalog # A07350-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PP11/ENDOU at approximately 47 kDa. The expected band size for PP11/ENDOU is at 47 kDa.
Anti-PP11/ENDOU Antibody Picoband(r)
A07350-1-10UG
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetENDOU
Overview
- SupplierBoster Bio
- Product NameAnti-PP11/ENDOU Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID8909
- Target nameENDOU
- Target descriptionendonuclease, poly(U) specific
- Target synonyms22 serine protease; 26 serine protease; endonuclease, polyU-specific; P11; placental protein 11; poly(U)-specific endoribonuclease; PP11; protein endoU; PRSS26; uridylate-specific endoribonuclease
- HostRabbit
- IsotypeIgG
- Protein IDP21128
- Protein NamePoly(U)-specific endoribonuclease
- Scientific DescriptionBoster Bio Anti-PP11/ENDOU Antibody Picoband® catalog # A07350-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203