Figure 1. Western blot analysis of PPP2CA using anti-PPP2CA antibody (PB9347). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: rat PC-12 whole cell lysates, Lane 8: mouse lung tissue lysates, Lane 9: mouse liver tissue lysates, Lane 10: mouse brain tissue lysates, Lane 11: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP2CA antigen affinity purified polyclonal antibody (Catalog # PB9347) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP2CA at approximately 34, 36 kDa. The expected band size for PPP2CA is at 36 kDa.
Figure 1. Western blot analysis of PPP2CA using anti-PPP2CA antibody (PB9347). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: rat PC-12 whole cell lysates, Lane 8: mouse lung tissue lysates, Lane 9: mouse liver tissue lysates, Lane 10: mouse brain tissue lysates, Lane 11: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP2CA antigen affinity purified polyclonal antibody (Catalog # PB9347) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP2CA at approximately 34, 36 kDa. The expected band size for PPP2CA is at 36 kDa.
Anti-PP2A-alpha/PPP2CA Antibody Picoband(r)
PB9347
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityChicken, Human, Mouse, Rat
TargetPPP2CA
Overview
- SupplierBoster Bio
- Product NameAnti-PP2A-Alpha Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityPP2A-alpha shares 100% cross reactivity with PPP2CB.
- Application Supplier NoteWB: The detection limit for PP2A-alpha is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID5515
- Target namePPP2CA
- Target descriptionprotein phosphatase 2 catalytic subunit alpha
- Target synonymsNEDLBA; PP2A-alpha; PP2Ac; PP2CA; PP2Calpha; protein phosphatase 2, catalytic subunit, alpha isozyme; replication protein C; RP-C; serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
- HostRabbit
- IsotypeIgG
- Protein IDP67775
- Protein NameSerine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
- Scientific DescriptionBoster Bio Anti-PP2A-alpha/PPP2CA Antibody Picoband® catalog # PB9347. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityChicken, Human, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203