Figure 1. Western blot analysis of PRMT3 using anti-PRMT3 antibody (A05694-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human Daudi whole cell lysates, Lane 7: human HL-60 whole cell lysates, Lane 8: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRMT3 antigen affinity purified polyclonal antibody (Catalog # A05694-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRMT3 at approximately 60-70 kDa. The expected band size for PRMT3 is at 60 kDa.
Figure 1. Western blot analysis of PRMT3 using anti-PRMT3 antibody (A05694-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human Daudi whole cell lysates, Lane 7: human HL-60 whole cell lysates, Lane 8: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRMT3 antigen affinity purified polyclonal antibody (Catalog # A05694-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRMT3 at approximately 60-70 kDa. The expected band size for PRMT3 is at 60 kDa.
Anti-PRMT3 Antibody Picoband(r)
A05694-1-10UG
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetPRMT3
Overview
- SupplierBoster Bio
- Product NameAnti-PRMT3 Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID10196
- Target namePRMT3
- Target descriptionprotein arginine methyltransferase 3
- Target synonymsheterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3; HMT1 hnRNP methyltransferase-like 3; HRMT1L3; protein arginine N-methyltransferase 3
- HostRabbit
- IsotypeIgG
- Protein IDO60678
- Protein NameProtein arginine N-methyltransferase 3
- Scientific DescriptionBoster Bio Anti-PRMT3 Antibody Picoband® catalog # A05694-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203