
Figure 1. Western blot analysis of PTGER1 using anti-PTGER1 antibody (PA1583). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGER1 antigen affinity purified polyclonal antibody (Catalog # PA1583) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTGER1 at approximately 42 kDa. The expected band size for PTGER1 is at 42 kDa.
Anti-Prostaglandin E Receptor EP1/PTGER1 Antibody Picoband(r)

PA1583
ApplicationsWestern Blot
Product group Antibodies
ReactivityHamster, Human
TargetPTGER1
Overview
- SupplierBoster Bio
- Product NameAnti-Prostaglandin E Receptor EP1 Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- Applications SupplierWB
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID5731
- Target namePTGER1
- Target descriptionprostaglandin E receptor 1
- Target synonymsEP1; PGE receptor, EP1 subtype; PGE2 receptor EP1 subtype; prostaglandin E receptor 1 (subtype EP1), 42kD; prostaglandin E receptor 1 (subtype EP1), 42kDa; prostaglandin E receptor 1, subtype EP1; prostaglandin E2 receptor EP1 subtype; prostanoid EP1 receptor
- HostRabbit
- IsotypeIgG
- Protein IDP34995
- Protein NameProstaglandin E2 receptor EP1 subtype
- Scientific DescriptionBoster Bio Anti-Prostaglandin E Receptor EP1/PTGER1 Antibody catalog # PA1583. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHamster, Human
- Reactivity SupplierHuman, Hamster
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203