
Figure 1. Western blot analysis of PRR9 using anti-PRR9 antibody (A14833). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRR9 antigen affinity purified polyclonal antibody (Catalog # A14833) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRR9 at approximately 13 kDa. The expected band size for PRR9 is at 13 kDa.
Anti-PRR9 Antibody Picoband(r)
A14833-PE
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetPRR9
Overview
- SupplierBoster Bio
- Product NameAnti-PRR9 Antibody Picoband(r)
- Delivery Days Customer9
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateRPE
- Gene ID574414
- Target namePRR9
- Target descriptionproline rich 9
- Target synonymsproline-rich protein 9
- HostRabbit
- IsotypeIgG
- Protein IDQ5T870
- Protein NameProline-rich protein 9
- Scientific DescriptionBoster Bio Anti-PRR9 Antibody Picoband® catalog # A14833. Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
References
- A conditional knockout resource for the genome-wide study of mouse gene function. Skarnes WC et al., 2011 Jun 15, NatureRead more