Anti-PV11 [III20]

Absolute Antibody

Anti-PV11 [III20]

7

The antibody binds the PV11 strain of the rabies virus.

The specificity of the scFv-Fc format of the antibody was confirmed by ELISA analysis. The scFv-Fc version of the antibody detected GPRV by western blot analysis. The scFv-Fc version of the antibody bound specifically to the membranes of PV11 infected Vero cells in an immunofluorescence assay. The scFv-Fc version of the antibody neutralized the PV11 strain in a standard in vivo neutralization assay where the virus was incubated with the scFv-Fc fragment before intracranial inoculation in mice (Ray et al., 2001; PMID: 11472431).

ImmunoFluorescence, Western Blot, ELISA, Neutralisation/Blocking

ELISA; WB; in vitro neutralization; IF

Research Use Only

Monoclonal

III20

Virus

Rabies virus

The original scFv antibody was selected from a human synthetic scFv phage display library by panning against the PV11 strain of the virus.

-20°C,2°C to 8°C

12352203