Figure 1. Western blot analysis of PYROXD1 using anti-PYROXD1 antibody (A17214-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PYROXD1 antigen affinity purified polyclonal antibody (Catalog # A17214-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PYROXD1 at approximately 65 kDa. The expected band size for PYROXD1 is at 50,56,72 kDa.
Figure 1. Western blot analysis of PYROXD1 using anti-PYROXD1 antibody (A17214-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PYROXD1 antigen affinity purified polyclonal antibody (Catalog # A17214-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PYROXD1 at approximately 65 kDa. The expected band size for PYROXD1 is at 50,56,72 kDa.
Anti-PYROXD1 Antibody Picoband(r)
A17214-4-BIOTIN
TargetPYROXD1
Product group Antibodies
Overview
- SupplierBoster Bio
- Product NameAnti-PYROXD1 Antibody Picoband(r)
- Delivery Days Customer9
- Concentration500 ug/ml
- Gene ID79912
- Target namePYROXD1
- Target descriptionpyridine nucleotide-disulphide oxidoreductase domain 1
- Target synonymsMFM8; pyridine nucleotide-disulfide oxidoreductase domain-containing protein 1
- Protein IDQ8WU10
- Protein NamePyridine nucleotide-disulfide oxidoreductase domain-containing protein 1
- Scientific DescriptionBoster Bio Anti-PYROXD1 Antibody Picoband® catalog # A17214-4. Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203