Anti-Rabbit IgG [A10B]

Absolute Antibody

Anti-Rabbit IgG [A10B]

7

Different formats of the antibody (scFv-cys, scFv, Fab and IgG) were employed for the construction of biosensors used for antigen detection in complex samples. In scFv-cys, one glycine of the linker was replaced with a cysteine, which determine an orientation of the scFv fragments on the sensor surface. The performance, in terms of sensitivity and selectivity, in biosensors prepared with scFv-cys, scFv, Fab and IgG was compared. The scFv-cys based biosensors displayed greater assay sensitivity and exhibited less nonspecific activity than intact monoclonal IgG and Fab fragment-based immunosensors. The scFv-cys biosensor surface was easily regenerated by stripping the rabbit IgG from the surface using an acidic solution. Moreover, western blot analysis showed purified scFv migrated as a single band at a MW of ~32 000 and did not appear to be degraded and the specificity of IgG1, Fab fragment and scFv towards rabbit IgG was determined by ELISA. (PMID: 15679346) In another study, two histidine amino acids were incorporated into the linker peptide of the scFv fragment and the scFv-His fragment was then employed to develop a new biosensor. The specificity of scFv and scFv-Hys were confirmed by ELISA. (PMID: 16255580) The affinity constant of rabbit IgG binding with scFv measured by using a conventional regeneration SPR method ((2.2 +/- 1.5) x 10^7 M-1) was comparable with the value determined with a nonregeneration protocol (2.5 +/- 0.2) x 10^7 M-1, and quartz crystal microbalance (1.9 x 10^7 M-1). (PMID: 16536419)

Western Blot, ELISA, Other Application

ELISA; WB; SPR

Research Use Only

-20°C,2°C to 8°C

12352203