Flow Cytometry analysis of HeLa cells using anti-Raf1 antibody (A00446-1). Overlay histogram showing HeLa cells stained with PB10089 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Raf1 Antibody (A00446-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Flow Cytometry analysis of HeLa cells using anti-Raf1 antibody (A00446-1). Overlay histogram showing HeLa cells stained with PB10089 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Raf1 Antibody (A00446-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Anti-Raf1 Antibody Picoband(r)
A00446-1
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRAF1
Overview
- SupplierBoster Bio
- Product NameAnti-Raf1 Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- ApplicationsFlow Cytometry, Western Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID5894
- Target nameRAF1
- Target descriptionRaf-1 proto-oncogene, serine/threonine kinase
- Target synonymsCMD1NN; CRAF; c-Raf; C-Raf proto-oncogene, serine/threonine kinase; NS5; Oncogene RAF1; proto-oncogene c-RAF; raf proto-oncogene serine/threonine protein kinase; RAF proto-oncogene serine/threonine-protein kinase; Raf-1; v-raf-1 murine leukemia viral oncogene homolog 1; v-raf-1 murine leukemia viral oncogene-like protein 1
- HostRabbit
- IsotypeIgG
- Protein IDP04049
- Protein NameRAF proto-oncogene serine/threonine-protein kinase
- Scientific DescriptionBoster Bio Anti-Raf1 Antibody Picoband® catalog # A00446-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203