Figure 1. Western blot analysis of Resistin using anti-Resistin antibody (PB9412). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Human Resistin Protein 0.5ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Resistin antigen affinity purified polyclonal antibody (Catalog # PB9412) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Resistin at approximately 11 kDa. The expected band size for Resistin is at 11 kDa.
Figure 1. Western blot analysis of Resistin using anti-Resistin antibody (PB9412). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Human Resistin Protein 0.5ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Resistin antigen affinity purified polyclonal antibody (Catalog # PB9412) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Resistin at approximately 11 kDa. The expected band size for Resistin is at 11 kDa.
Anti-Resistin/RETN Antibody Picoband(r)
PB9412-BIOTIN
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetRETN
Overview
- SupplierBoster Bio
- Product NameAnti-Resistin/RETN Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteWB: The detection limit for Resistin is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateBiotin
- Gene ID56729
- Target nameRETN
- Target descriptionresistin
- Target synonymsadipose tissue-specific secretory factor; ADSF; C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 1; c/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; cysteine-rich secreted protein A12-alpha-like 2; cysteine-rich secreted protein FIZZ3; FIZZ3; found in inflammatory zone 3; resistin; resistin delta2; RETN1; RSTN; XCP1
- HostRabbit
- IsotypeIgG
- Protein IDQ9HD89
- Protein NameResistin
- Scientific DescriptionBoster Bio Anti-Resistin/RETN Antibody Picoband® catalog # PB9412. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203