Figure 1. Western blot analysis of RIOK3 using anti-RIOK3 antibody (A09262-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIOK3 antigen affinity purified polyclonal antibody (Catalog # A09262-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RIOK3 at approximately 65 kDa. The expected band size for RIOK3 is at 59 kDa.
Figure 1. Western blot analysis of RIOK3 using anti-RIOK3 antibody (A09262-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIOK3 antigen affinity purified polyclonal antibody (Catalog # A09262-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RIOK3 at approximately 65 kDa. The expected band size for RIOK3 is at 59 kDa.
Anti-RIOK3 Antibody Picoband(r)
A09262-3-FITC
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetRIOK3
Overview
- SupplierBoster Bio
- Product NameAnti-RIOK3 Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsFlow Cytometry, Western Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateFITC
- Gene ID8780
- Target nameRIOK3
- Target descriptionRIO kinase 3
- Target synonymshomolog of the Aspergillus nidulans sudD gene product; serine/threonine-protein kinase RIO3; SUDD; sudD (suppressor of bimD6, Aspergillus nidulans) homolog; sudD homolog; sudD suppressor of Aspergillus nidulans bimD6 homolog; sudD suppressor of bimD6 homolog; testicular secretory protein Li 47
- HostRabbit
- IsotypeIgG
- Protein IDO14730
- Protein NameSerine/threonine-protein kinase RIO3
- Scientific DescriptionBoster Bio Anti-RIOK3 Antibody Picoband® catalog # A09262-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203