Anti-RNA polymerase II [CTD 4H8]

Absolute Antibody

Anti-RNA polymerase II [CTD 4H8]

9

CTD 4H8 is known to be reactive with human and S. cerevisiae RNA polymerase II CTDs, however may also be cross-reactive with this protein in other species, including mouse, rat and chicken. The Ab is also reactive with both the phosphorylated and unphosph

This mAb has been used in a variety of methods, including Western blotting of the unphosphorylated (Engelhardt et al, 2005) or Ser5-CTD phosphorylated form (Stock et al, 2007), and in precipitation experiments either simply pulling down RNAPII (Kukalev et al, 2005; Peng et al, 2014) or coupled to massively parallel DNA sequencing (ChIP-seq) of the total RNAPII-associated DNA isolated from human CD4+ cells (Zhang et al, 2012).

Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoHistoChemistry

Research Use Only

Monoclonal

CTD 4H8

POLR2A

RNA polymerase II subunit A

DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; hRPB220; hsRPB1; NEDHIB; POLR2; POLRA; polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; polymerase (RNA) II subunit A; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RPB1; RPBh1; RpIILS; RPO2; RPOL2

Human

IgG4

DNA-directed RNA polymerase II subunit RPB1

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human, Yeast

-20°C,2°C to 8°C

12352203