Figure 1. Western blot analysis of ROC1 using anti-ROC1 antibody (PB9798). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: Rat Testis Tissue Lysate, Lane 2: Rat Brain Tissue Lysate, Lane 3: Mouse Brain Tissue Lysate, Lane 4: Mouse Spleen Tissue Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROC1 antigen affinity purified polyclonal antibody (Catalog # PB9798) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROC1 at approximately 15 kDa. The expected band size for ROC1 is at 15 kDa.
Figure 1. Western blot analysis of ROC1 using anti-ROC1 antibody (PB9798). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: Rat Testis Tissue Lysate, Lane 2: Rat Brain Tissue Lysate, Lane 3: Mouse Brain Tissue Lysate, Lane 4: Mouse Spleen Tissue Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROC1 antigen affinity purified polyclonal antibody (Catalog # PB9798) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROC1 at approximately 15 kDa. The expected band size for ROC1 is at 15 kDa.
Anti-ROC1/RBX1 Antibody Picoband(r)
PB9798-DYLIGHT488
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHamster, Human, Mouse, Rat
TargetRBX1
Overview
- SupplierBoster Bio
- Product NameAnti-ROC1/RBX1 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateDyLight 488
- Gene ID9978
- Target nameRBX1
- Target descriptionring-box 1
- Target synonymsBA554C12.1; E3 ubiquitin-protein ligase RBX1; E3 ubiquitin-protein transferase RBX1; regulator of cullins 1; RING finger protein 75; ring-box 1, E3 ubiquitin protein ligase; RING-box protein 1; RNF75; ROC1; ZYP protein
- HostRabbit
- IsotypeIgG
- Protein IDP62877
- Protein NameE3 ubiquitin-protein ligase RBX1
- Scientific DescriptionBoster Bio Anti-ROC1/RBX1 Antibody Picoband® catalog # PB9798. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHamster, Human, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203