Western blot analysis of RPA1 expression in HEK293 cell lysate using anti-RPA1 antibody (M01317-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPA1 antigen affinity purified polyclonal antibody (Catalog # M01317-1) at 0.5 ug/mL overnight at 4
Western blot analysis of RPA1 expression in HEK293 cell lysate using anti-RPA1 antibody (M01317-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPA1 antigen affinity purified polyclonal antibody (Catalog # M01317-1) at 0.5 ug/mL overnight at 4
Anti-RPA70 Monoclonal Antibody
M01317-1
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRPA1
Overview
- SupplierBoster Bio
- Product NameAnti-RPA70 Monoclonal Antibody
- Delivery Days Customer9
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDBB-18
- FormulationLiquid
- Gene ID6117
- Target nameRPA1
- Target descriptionreplication protein A1
- Target synonymsHSSB; MST075; MSTP075; REPA1; replication factor A protein 1; replication protein A 70 kDa DNA-binding subunit; replication protein A1, 70kDa; RF-A; RF-A protein 1; RP-A; RP-A p70; RPA70; single-stranded DNA-binding protein
- HostRabbit
- IsotypeIgG
- Protein IDP27694
- Protein NameReplication protein A 70 kDa DNA-binding subunit
- Scientific DescriptionBoster Bio Anti-RPA70 Monoclonal Antibody catalog # M01317-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
- ReactivityHuman
- Storage Instruction-20°C
- UNSPSC12352203