Figure 1. Western blot analysis of RPL38 using anti-RPL38 antibody (A10156-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPL38 antigen affinity purified polyclonal antibody (Catalog # A10156-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPL38 at approximately 17 kDa. The expected band size for RPL38 is at 8 kDa.
Figure 1. Western blot analysis of RPL38 using anti-RPL38 antibody (A10156-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPL38 antigen affinity purified polyclonal antibody (Catalog # A10156-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPL38 at approximately 17 kDa. The expected band size for RPL38 is at 8 kDa.
Anti-RPL38 Antibody Picoband(r)
A10156-1-DYLIGHT550
TargetRPL38
Product group Antibodies
Overview
- SupplierBoster Bio
- Product NameAnti-RPL38 Antibody Picoband(r)
- Delivery Days Customer9
- Concentration500 ug/ml
- Gene ID6169
- Target nameRPL38
- Target descriptionribosomal protein L38
- Target synonyms60S ribosomal protein L38; L38; large ribosomal subunit protein eL38
- Protein IDP63173
- Protein Name60S ribosomal protein L38
- Scientific DescriptionBoster Bio Anti-RPL38 Antibody Picoband® catalog # A10156-1. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203