
Figure 1. Western blot analysis of RTEL1 using anti-RTEL1 antibody (A01544-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RTEL1 antigen affinity purified polyclonal antibody (Catalog # A01544-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RTEL1 at approximately 160 kDa. The expected band size for RTEL1 is at 134 kDa.
Anti-RTEL1 Antibody Picoband(r)
A01544-2-10UG
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRTEL1
Overview
- SupplierBoster Bio
- Product NameAnti-RTEL1 Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsFlow Cytometry, Western Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID51750
- Target nameRTEL1
- Target descriptionregulator of telomere elongation helicase 1
- Target synonymsC20orf41; DKCA4; DKCB5; NHL; PFBMFT3; regulator of telomere elongation helicase 1; regulator of telomere length; RTEL
- HostRabbit
- IsotypeIgG
- Protein IDQ9NZ71
- Protein NameRegulator of telomere elongation helicase 1
- Scientific DescriptionBoster Bio Anti-RTEL1 Antibody Picoband® catalog # A01544-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203