Figure 1. Western blot analysis of RTL10 using anti-RTL10 antibody (A31740). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human A431 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RTL10 antigen affinity purified polyclonal antibody (Catalog # A31740) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for RTL10 at approximately 36-39KD. The expected band size for RTL10 is at 39KD.
Figure 1. Western blot analysis of RTL10 using anti-RTL10 antibody (A31740). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human A431 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RTL10 antigen affinity purified polyclonal antibody (Catalog # A31740) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for RTL10 at approximately 36-39KD. The expected band size for RTL10 is at 39KD.
Anti-RTL10 Antibody Picoband(r)
A31740-DYLIGHT594
TargetRTL10
Product group Antibodies
Overview
- SupplierBoster Bio
- Product NameAnti-RTL10 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- Concentration500 ug/ml
- Gene ID79680
- Target nameRTL10
- Target descriptionretrotransposon Gag like 10
- Target synonymsBH3-only protein; BOP; C22orf29; protein Bop; retrotransposon Gag-like protein 10
- Protein IDQ7L3V2
- Protein NameProtein Bop
- Scientific DescriptionBoster Bio Anti-RTL10 Antibody Picoband® catalog # A31740. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203