Anti-S protein (RBD) [5F8]

Absolute Antibody

Anti-S protein (RBD) [5F8]

9

The antibody binds the receptor binding domain (RBD) of the SARS-CoV-2 Spike protein.

The binding affinity of the VHH fragment towards SARS-CoV-2 or SARC-CoV RBD was measured using surface plasmon resonance (Kd= 0.996 and 239,2 nM respectively). The neutralization activity of the VHH fragment with SARS-CoV-2 pseudotypes or SARS-CoV-2 was tested, the showing half maximal neutralization concentration (EC50) of 0.0009 microg/mL and 0.51 microg/mL respectively. Competition-binding assay showed the clone could just partially prevent binding of SARS-CoV-2 RBD to ACE2. Neutralization assay of the bivalent form of the clone fused with human IgG1 Fc against SARS-CoV-2pp was performed, showing EC50= 0.004 microg/mL (Chi et al, 2020; pmid: 32913273).

Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

5F8

S

surface glycoprotein

GU280_gp02; spike glycoprotein; surface glycoprotein

Human

IgG1

Spike glycoprotein

This chimeric human antibody was made using the variable domain sequences of the original VHH format for improved compatibility with existing reagents assays and techniques.

Virus

-20°C,2°C to 8°C

12352203