Anti-S100A8 & S100A9 [5.5]

Absolute Antibody

Anti-S100A8 & S100A9 [5.5]

9

mAb 5.5 specifically recognises complexed MRP-8/MRP-14 via the hydrophobic residues in helix IV of MRP-8 (S100A8) exposed during heterodimer formation. mAb recognition of MRP-8 & MRP-14 is calcium dependent. 5.5 epitope is most likely to be formed by line

mAb 5.5 has been used in immunoprecipitation and western blot experiments for the characterisation of p8,14 identified in extracts of monocytes and neutrophils. 5.5 was also used in flow cytometric analysis to show that p8,14 is not a cell surface protein, and in immunohistochemical staining to show that p8,14 is present extracellularly (Edgeworth 1991).

Flow Cytometry, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry

Research Use Only

Monoclonal

5.5

S100A8

S100 calcium binding protein A8

60B8AG; CAGA; calgranulin A; calprotectin L1L subunit; CFAG; CGLA; CP-10; cystic fibrosis antigen; L1Ag; leukocyte L1 complex light chain; MA387; MIF; migration inhibitory factor-related protein 8; MRP8; NIF; P8; protein S100-A8; S100-A8; urinary stone protein band A

Human

IgG1

Protein S100-A8

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

12352203