Anti-SARS-CoV S glycoprotein [1B5]

Absolute Antibody

Anti-SARS-CoV S glycoprotein [1B5]

9

This antibody specifically binds the amino acids 318-510 in the S1 domain of the SARS-CoV Spike protein (Urbani strain).

This antibody inhibits the association of SARS-CoV s protein to the angiotensin-converting enzyme 2 (ACE2) receptor by binding to specific amino acids on the S1 domain of the spike protein of the virus. The originally characterized antibody had a neutralizing titer 200TCID50 of 12.5 microg/ml (Coughlin et at.,2006) and IC50 of 2.84 microg/ml when incubated with S12-510- Fc recombinant protein (Coughlin et at.,2009). Purified 1B5 binds to RBD region with a unique CDR3 sequence. When used in combination with 4D4 the lack of synergistic effect indicated that the mAbs 4D4 and 1B5 likely interact with closely positioned conformational epitopes and compete with each other for binding (Coughlin et at.,2009). ELISA.

ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

1B5

Mouse

IgG2b

Spike glycoprotein

This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG2 format, for improved compatibility with existing reagents, assays and techniques.

Virus

-20°C,2°C to 8°C

12352203