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Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells of tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells of tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells of tubules.

Anti-SASS6 Antibody

HPA028187
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSASS6
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SASS6 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID163786
  • Target name
    SASS6
  • Target description
    SAS-6 centriolar assembly protein
  • Target synonyms
    MCPH14, SAS-6, SAS6, spindle assembly abnormal protein 6 homolog, spindle assembly defective protein 6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6UVJ0
  • Protein Name
    Spindle assembly abnormal protein 6 homolog
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of SASS6 using anti-SASS6 antibody (A05762-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SASS6 antigen affinity purified polyclonal antibody (Catalog # A05762-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SASS6 at approximately 74,70-74 kDa. The expected band size for SASS6 is at 74 kDa.
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