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Western blot analysis of SCF expression in recombinant protein (M01254). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KITLG monoclonal antibody (Catalog # M01254) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KITLG
Western blot analysis of SCF expression in recombinant protein (M01254). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KITLG monoclonal antibody (Catalog # M01254) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KITLG
Western blot analysis of SCF expression in recombinant protein (M01254). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KITLG monoclonal antibody (Catalog # M01254) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KITLG

Anti-SCF Rabbit Monoclonal Antibody

Research Use Only
M01254
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetKITLG
Price on request
100 ul
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-SCF Rabbit Monoclonal Antibody
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    FAG-11
  • Formulation
    Liquid
  • Gene ID4254
  • Target name
    KITLG
  • Target description
    KIT ligand
  • Target synonyms
    c-Kit ligand; DCUA; DFNA69; familial progressive hyperpigmentation 2; FPH2; FPHH; kit ligand; Kitl; KL-1; mast cell growth factor; MGF; SCF; SF; SHEP7; SLF; steel factor; stem cell factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP21583
  • Protein Name
    Kit ligand
  • Scientific Description
    Boster Bio Anti-SCF Rabbit Monoclonal Antibody catalog # M01254. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203