Anti-SEB [20B1]

Absolute Antibody

Anti-SEB [20B1]

9

This antibody binds a conformational epitope near the C terminal end of the Staphylococcal enterotoxin B. It is suspected that this antibody reacts with 135-R, 137-F, 186-Y, 235- and 236-T of the enterotoxin. This antibody also cross reacts with superanti

This antibody in combination with 6D3 and 14G8 can be used to detect Staphylococcal enterotoxin B (SEB) in a sample using capture ELISA. This antibody is capable of detecting SEB in a western blot assay but not staphylococcal enterotoxin A (SEA) or toxic shock syndrome toxin 1 (TSST-1). 20B1 demonstrated comparable level of inhibition of SEB incuded T cell-proliferation in human T-cells from a normal donor. 20B1 also caused inhibition of cytokine induction in treated T-cells as they produced less IFN-gamma and IL-2. It was reported that monotherapy with 20B1 alone is capable of neutralizing SEB and provided protection against SEB induced lethal shock (SEBILS) in BALB/c mice and HLA-DR3 transgenic mouse model. It was also recommended that combination with mAb 14G8 or 6D3 enhanced protection and showed better results (PMID: 21233204). In vivo studies indicated that the IgG2a isotype variant of 20B1 exhibited significantly greater protection than IgG1 or IgG2b in murine SEB intoxication and S. aureus sepsis models (PMID: 24917594). ELISA assays confirmed binding of mAb 20B1 to SEC-1, and the binding affinity obtained from BLI analysis was 2.5 +/- 0.45 nM. Superantigens SEC-2, SEC-3 and streptococcal superantigen A (SSA) did not bind mAb 20B1 (PMID: 25572397).

Western Blot, ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

20B1

Rabbit

IgG

Enterotoxin type B

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Bacteria

-20°C,2°C to 8°C

12352203