
Figure 1. Western blot analysis of SENP1 using anti-SENP1 antibody (A02156-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human HEPG2 whole cell lysates, Lane 3: human HELA whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human U87 whole cell lysates, Lane 6: human Raji whole cell lysates, Lane 7: human HL-60 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SENP1 antigen affinity purified polyclonal antibody (Catalog # A02156-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SENP1 at approximately 73KD. The expected band size for SENP1 is at 73KD.
Anti-SENP1 Antibody Picoband(r)
A02156-3-BIOTIN
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetSENP1
Overview
- SupplierBoster Bio
- Product NameAnti-SENP1 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- ApplicationsFlow Cytometry, Western Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateBiotin
- Gene ID29843
- Target nameSENP1
- Target descriptionSUMO specific peptidase 1
- Target synonymssentrin/SUMO-specific protease SENP1; sentrin-specific protease 1; SUMO1/sentrin specific peptidase 1; SUMO1/sentrin specific protease 1; SuPr-2
- HostRabbit
- IsotypeIgG
- Protein IDQ9P0U3
- Protein NameSentrin-specific protease 1
- Scientific DescriptionBoster Bio Anti-SENP1 Antibody Picoband® catalog # A02156-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203