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Figure 1. Western blot analysis of SENP1 using anti-SENP1 antibody (A02156-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human HEPG2 whole cell lysates, Lane 3: human HELA whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human U87 whole cell lysates, Lane 6: human Raji whole cell lysates, Lane 7: human HL-60 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SENP1 antigen affinity purified polyclonal antibody (Catalog # A02156-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SENP1 at approximately 73KD. The expected band size for SENP1 is at 73KD.
Figure 1. Western blot analysis of SENP1 using anti-SENP1 antibody (A02156-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human HEPG2 whole cell lysates, Lane 3: human HELA whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human U87 whole cell lysates, Lane 6: human Raji whole cell lysates, Lane 7: human HL-60 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SENP1 antigen affinity purified polyclonal antibody (Catalog # A02156-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SENP1 at approximately 73KD. The expected band size for SENP1 is at 73KD.
Figure 1. Western blot analysis of SENP1 using anti-SENP1 antibody (A02156-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human HEPG2 whole cell lysates, Lane 3: human HELA whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human U87 whole cell lysates, Lane 6: human Raji whole cell lysates, Lane 7: human HL-60 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SENP1 antigen affinity purified polyclonal antibody (Catalog # A02156-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SENP1 at approximately 73KD. The expected band size for SENP1 is at 73KD.

Anti-SENP1 Antibody Picoband(r)

A02156-3-BIOTIN
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetSENP1
100 ug
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-SENP1 Antibody Picoband(r)
  • Delivery Days Customer
    9
  • Antibody Specificity
    No cross reactivity with other proteins.
  • Applications
    Flow Cytometry, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    Biotin
  • Gene ID29843
  • Target name
    SENP1
  • Target description
    SUMO specific peptidase 1
  • Target synonyms
    sentrin/SUMO-specific protease SENP1; sentrin-specific protease 1; SUMO1/sentrin specific peptidase 1; SUMO1/sentrin specific protease 1; SuPr-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9P0U3
  • Protein Name
    Sentrin-specific protease 1
  • Scientific Description
    Boster Bio Anti-SENP1 Antibody Picoband® catalog # A02156-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203