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Anti-SERT [8B6]

Ab00943-23.0
Absolute Antibody
ApplicationsOther Application
Product group Antibodies
TargetSLC6A4
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-SERT [8B6]
  • Delivery Days Customer
    7
  • Application Supplier Note
    The 8B6 Fab fragment was crystallised in complex with human SERT to aid structure determination (Coleman et al, 2016).
  • Applications
    Other Application
  • Applications Supplier
    Crystallisation
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    8B6
  • Gene ID6532
  • Target name
    SLC6A4
  • Target description
    solute carrier family 6 member 4
  • Target synonyms
    5HT transporter; 5HTT; 5-HTT; 5-HTTLPR; 5-hydroxytryptamine (serotonin) transporter; hSERT; HTT; Na+/Cl- dependent serotonin transporter; OCD1; serotonin transporter 1; SERT; SERT1; sodium-dependent serotonin transporter; solute carrier family 6 (neurotransmitter transporter), member 4; solute carrier family 6 (neurotransmitter transporter, serotonin), member 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP31645
  • Protein Name
    Sodium-dependent serotonin transporter
  • Scientific Description
    This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    8B6 was produced by immunising SERT KO mice with residues 73-616 of wildtype human SERT. Hybridomas were generated using established protocols.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SLC6A4 using anti-SLC6A4 antibody (PB9438). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human COLO-320 whole cell lysates. Lane 5: human K562 whole cell lysates, Lane 6: human U2OS whole cell lysates, Lane 7: human U-87MG whole cell lysates, Lane 8: rat heart tissue lysates, Lane 9: mouse heart tissue lysates, Lane 10: mouse SP20 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC6A4 antigen affinity purified polyclonal antibody (Catalog # PB9438) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC6A4 at approximately 90KD. The expected band size for SLC6A4 is at 70KD.
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